Development of an Efficient Targeted Cell-SELEX Procedure for DNA Aptamer Reagents

DNA aptamers generated by cell-SELEX offer an attractive alternative to antibodies, but generating aptamers to specific, known membrane protein targets has proven challenging, and has severely limited the use of aptamers as affinity reagents for cell identification and purification. We modified the BJAB lymphoblastoma cell line to over-express the murine c-kit cell surface receptor.

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