From the Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA; and Division of Cardiovascular Medicine, Brigham and Women's Hospital, Boston, MA. present the use of a novel clustered regularly interspaced short palindromic repeat -CRISPR-associated 9 method to inactivate the calponin-1 gene involved in smooth muscle contraction, by introducing a knockin mutation to cleanly disrupt a CC 6GG element in the first intron of the gene.
http://ift.tt/1JO8sM3
http://ift.tt/1JO8sM3
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